Experiments in Plant Tissue Culture

Description

In this third edition the authors have written a guide to the essentials of plant tissue culture. The book takes the reader through a graded series of experimental protocols and provides an introductory review of each topic. There are discussions of aseptic techniques, and nutritional components of media. Subsequent chapters cover such topics as callus formation, organogenesis, xylem cell differentiation, root cultures, cell suspensions, micropropagation by buds, somatic embryogenesis, protoplast culture and fusion, secondary metabolite production, and quantitation of procedures. A glossary, commercial sources of supplies, and the formulations of culture media are also included. This new edition has been completely revised and updated. The organization has been improved, and new illustrations have been added, together with new experiments on such topics as potato callus formation, and embryo culture. This book will be useful to graduate students and professionals in plant science, forestry, agronomy, and horticulture.

'This well thought out book does considerably more than is claimed in the title. It not only takes the reader through a graded series of experimental protocols covering the major techniques used in plant tissue culture, it also provides a good introductory review of each topic.' Trends in Biochemical Sciences '... the authors have written ... a text based on their extensive experience ... directed in the first instance towards students, these topics will prove [to be] of immense value to a much wider range of readers, whatever their previous knowledge.' Cellular and Molecular Biology '...comprehensive and yet concise...Invaluable to all those interested or involved in the area of plant tissue culture and commends itself as an important source of information about techniques used in a wide range of research and teaching institutions as well as in commercial companies. This excellent book achieves much more than its title suggests.' G. Ben-Hod, Israel Journal of Plant Science '... a worthy text and an excellent addition to any plant science laboratory.' John Love, British Society for Developmental Biology Newsletter